Identification of new dermaseptins with self-assembly tendency: membrane disruption, biofilm eradication, and infected wound healing efficacy

Journal:Acta Biomaterialia

Funded by:国家自然科学基金项目

Key Words:Antimicrobial peptides、Molecular cloning、 Biofilm 、MRSA infection 、Aggregation

Abstract:Severe infections associated with antibiotic-resistant bacteria and biofilms have attracted increasing interest as these diseases are difficult to treat with current antibiotics. Typical cationic antimicrobial peptides dermaseptins are considered to be the most promising next-generation antibiotics because of their broadspectrum antimicrobial activities and minor side effects. Two new dermaseptin peptides, DMS-PS1 and DMS-PS2, have been identified by “shotgun” molecular cloning of encoding cDNAs in the crude skin secretions of the waxy monkey tree frog, Phyllomedusa sauvagei. The mature peptide sequences predictedfrom the cloned cDNAs were separated from crude skin secretions and confirmed by mass spectrometry.Chemically synthetic replicates were assessed for various biological activities. Both dermaseptins were potently effective against a broad spectrum of microorganisms including antibiotic-resistant bacteria and displayed significant potency against gram-positive and gram-negative bacterial biofilms with low toxicity towards mammalian red blood cells. Remarkably, DMS-PS2 was effective against infections in murine skin caused by methicillin-resistant Staphylococcus aureus as a result of an induced wound. The actions of DMS-PS2 were with a membrane permeabilization mode. Overall, the data provided convincing evidence for the development of anti-infectious agents and/or biomaterials as a new therapeutic approach against bacterial infections.

Co-author:SD

Correspondence Author:高艺恬

Volume:109

Number of Words:6

ISSN No.:1050-4648

Translation or Not:no

CN No.:doi.org/10.1016/j.actbio.2020.03.024

Date of Publication:2020-04-08